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Catalogue Number : AB9046
Qty/Pk : 100 µL

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Sheep anti-Myelin Basic Protein (AB9046)

Anti-Myelin Basic Protein

Species Reactivity	Key Applications	Host	Format	Antibody Type
B, H, M	WB, IH	Sheep	Serum	Polyclonal Antibody

UniProt Number:

P02686

UniProt Summary

FUNCTION: SwissProt: P02686 # The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, each of them having maybe a specialized function. Induces T-cell proliferation.
SIZE: 304 amino acids; 33117 Da
SUBUNIT: Homodimer; isoform 3 exists as a homodimer.
SUBCELLULAR LOCATION: Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.
TISSUE SPECIFICITY: MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.DEVELOPMENTAL STAGE: Expression turns on abruptly in fetus of 14 to 16 weeks. Even smaller isoforms seem to be produced during embryogenesis, some of these persisting in the adult. Expression of isoform MBP2 is more evident at 16 weeks and its relative proportion declined thereafter.
PTM: Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases in aging, making the protein more cationic. & The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6). & Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
DISEASE: SwissProt: P02686 # The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
SIMILARITY: SwissProt: P02686 ## Belongs to the myelin basic protein family. ... see more »

Entrez Gene Number:

NM_001025094.1

Entrez Gene Summary

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP ... see more »

Description:

Anti-Myelin Basic Protein

Alternate Names:

MBP

Specificity:

Reacts with Myelin Basic Protein (MBP). May show reactivity with golli proteins. By western blots the antibody shows reactivity to all four isoforms of MBP in human, mouse and bovine species tested.

Key Applications:

Western Blotting
Immunohistochemistry

Application Notes:

Western blot: 1:5,000-1:10,000. The antibody recognizes bands at 14, 17, 18.5 and 21.5 kDa

Immunohistochemistry: 1:6,000-1:12,000 on frozen mouse cerebellar white matter.

Optimal working dilutions must be determined by the end user.

Species Reactivity:

Bovine
Human
Mouse

Immunogen:

Purified Bovine 18.5 MBP protein

Presentation:

Sheep serum. Liquid containing no preservative.

Storage Conditions:

Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Gene Symbol:

MBP
MGC99675
Golli-mbp

Antibody Category:

Neuroscience

Antibody Sub-Category:

Neuronal & Glial Markers
Neurochemistry & Neurotrophins

Usage Statement:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Trade Name:

Chemicon (Millipore)

Format:

Serum

Host:

Sheep

Antibody Type:

Polyclonal Antibody